Fig. 1.

(a) DIC set-up used to obtain images of the medial side of the tibia (Adapted from (Sztefek et al., 2010)), (b) diagrams showing the two loading positions tested. In Position 1 the bone is axially loaded, whereas in Position 2 the bone is loaded at a 30° flexure respect to the vertical axis, and (c) transversal view of the bottom loading cup showing the position of the mouse foot (the top cup at the knee was also rotated at the same amount) when the right tibia was loaded at 0°, −45° and +45° rotation angle with respect to the CCD camera axis. Rotation 1 refers to the position of the medial surface of the tibia when parallel to cameras, Rotation 2 refers to the position of the medial surface of the tibia at −45° with respect to cameras and finally Rotation 3 refers to the position of the medial surface of the tibia at 45° respect to the camera axis.