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. Author manuscript; available in PMC: 2014 Jun 26.
Published in final edited form as: J Pathol. 2013 Oct;231(2):199–209. doi: 10.1002/path.4207

Figure 5.

Figure 5

Analysis of OPN expression in muscles injected with either ssRNA or saline. (A) Real-time PCR was performed on cDNA synthesized from RNA extracted from muscle samples injected with either saline or ssRNA in WT, A/J, and double-deficient muscles. The Ct values were used to calculate OPN gene expression in ssRNA-treated muscles normalized to HPRT, relative to the normalized level of OPN expression in saline-treated muscles. (B) ELISA detection of OPN levels in saline- and ssRNA-injected muscles of A/J mice. Data are presented as means ± SEM (n = 4 animals from each treatment and genotype). *p ≤ 0.05 and ***p ≤ 0.001; the randomization reallocation test was used for comparison of ssRNA-treated and saline-treated muscles in A. Unpaired Student’s t-test was used for comparisons in B.