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. 2014 Jul 22;281(1787):20140619. doi: 10.1098/rspb.2014.0619

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Immuno-assay by western blotting demonstrates internalization of Hv1a/GNA in adult honeybee tissues. Bands of GNA (12 kDa) and Hv1a/GNA (FP; 16 kDa) are indicated. (a) Diagram of adult honeybee showing the presence of GNA and fusion protein Hv1a/GNA (FP) in both the haemolymph and brain after feeding solutions containing proteins. Insects were fed 100 µg GNA or Hv1a/GNA, and haemolymph or brain tissue was collected after 24 h for analysis. (b) Diagram of larval honeybee showing that Hv1a/GNA (FP) is degraded after ingestion; larvae were dosed with 100 µg Hv1a/GNA per larva and haemolymph was collected after 24 h for analysis.