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. 2014 Jun 26;8:179. doi: 10.3389/fncel.2014.00179

Figure 1.

Figure 1

Depletion of CIIA by RNAi potentiates SOD1(G93A)-induced cytotoxicity. (A) Neuro2a (N2a) cells expressing Flag-tagged human SOD1(WT) or SOD1(G93A) were transfected for 48 h with si RNAs specific for GFP or CIIA mRNAs. The cells were stained with annexin V and PI, and the percentages of apoptotic cells were determined by flow cytometry. (B) NSC34 cells expressing Flag-tagged SOD1(WT) or SOD1(G93A) were transfected for 48 h with plasmids encoding RFP along with GFP (control) or CIIA siRNA. The cells were then examined for apoptosis by TUNEL assay. Left, representative images of the assay (scale bar, 100 μm). Arrows indicate TUNEL-positive cells. Right, the data are means ± s.e.m. from three independent experiments.