Figure 2.

CIIA inhibits SOD1(G93A)-induced activation of ASK1. NSC34 cells cells were stably transfected with plasmid vectors encoding Flag-tagged SOD1(WT) or SOD1(G93A) along with vectors for GFP or CIIA siRNA. (A,B) Cell lysates of the stable transfectants were examined for ASK1 and p38 kinase activities by immune complex kinase assay (A) or for intracellular ROS production by DCF fluorescence (B). The data are means ± s.e.m. from three independent experiments. ^*P < 0.05 vs. WT. (C) Cell lysates were subjected to immunoprecipitation with antibodies to ASK1, and the resulting precipitates were immunoblotted with antibodies to TRAF2 or to CIIA.