Table 1.
Physiological parameters of P. multiseries and P. delicatissima cells grown without added bacteria (M0 and D0, respectively), with its own bacteria (MM and DD, respectively) or with bacteria from the other Pseudo-nitzschia species (MD and DM, respectively). Percentage of active cells and esterase activity were measured after FDA staining and lipid content after BODIPY staining, the latter two expressed in arbitrary units.
| Physiological Parameter | Age of Culture (Days) | Mean ± SE | Mean ± SE | Mean ± SE |
| P. multiseries | ||||
| M0 | MM | MD | ||
| Percentage of Active Cells | 3 | 76.9 ± 2.0 | 73.9 ± 1.2 | 81.1 ± 0.8 |
| 4 | 97.6 ± 1.4 | 95.5 ± 1.1 | 93.6 ± 1.5 | |
| 5 | 97.2 ± 0.8 | 98.3 ± 0.4 | 95.8 ± 1.0 | |
| 7 | 97.3 ± 0.2 | 96.6 ± 0.5 | 95.2 ± 0.4 | |
| 8 | 96.7 ± 0.4 | 95.7 ± 0.2 | 93.2 ± 0.8 | |
| 10 | 97.3 ± 0.4 | 97.7 ± 0.1 | 95.0 ± 0.7 | |
| 11 | 97.6 ± 0.4 | 98.0 ± 0.2 | 92.8 ± 1.5 | |
| 12 | 97.5 ± 0.2 | 97.0 ± 0.5 | 92.1 ± 1.2 | |
| 14 | 97.3 ± 0.3 | 98.1 ± 0.2 | 90.7 ± 1.5 | |
| 15 | 96.5 ± 0.2 | 98.1 ± 0.7 | 92.8 ± 0.7 | |
| 17 | 94.4 ± 0.4 | 96.2 ± 1.2 | 85.9 ± 2.9 | |
| M0 | MM | MD | ||
| Esterase Activity | 3 | 130.0 ± 7.8 | 149.3 ± 13.2 | 138.7 ± 20.8 |
| 4 | 157.3 ± 37.3 | 132.7 ± 11.4 | 167.2 ± 27.7 | |
| 5 | 76.8 ± 45.5 | 114.2 ± 8.5 | 187.1 ± 15.3 | |
| 7 | 122.5 ± 8.3 | 139.5 ± 6.4 | 198.5 ± 21.5 | |
| 8 | 116.3 ± 3.6 | 129.4 ± 7.9 | 159.9 ± 7.4 | |
| 10 | 121.6 ± 1.6 | 148.9 ± 9.0 | 145.2 ± 15.1 | |
| 11 | 112.4 ± 5.1 | 137.3 ± 8.7 | 148.5 ± 9.0 | |
| 12 | 111.1 ± 1.5 | 128.7 ± 9.7 | 141.9 ± 9.9 | |
| 14 | 126.6 ± 9.2 | 161.6 ± 7.6 | 128.5 ± 15.0 | |
| 15 | 106.2 ± 7.4 | 140.7 ± 11.5 | 114.1 ± 7.7 | |
| 17 | 131.9 ± 15.6 | 174.0 ± 13.8 | 111.9 ± 15.6 | |
| M0 | MM | MD | ||
| Lipid Content | 3 | 1466.4 ± 65.6 | 1443.7 ± 42.7 | 1303.8 ± 48.4 |
| 4 | 1516.1 ± 123.7 | 1572.0 ± 212.6 | 1493.2 ± 293.8 | |
| 5 | 1530.4 ± 83.2 | 1336.8 ± 171.1 | 1353.7 ± 23.5 | |
| 7 | 1099.5 ± 123.6 | 1099.4 ± 255.4 | 1147.7 ± 38.0 | |
| 8 | 1121.6 ± 63.7 | 1193.9 ± 67.1 | 1050.7 ± 17.1 | |
| 10 | 1013.9 ± 41.5 | 1092.6 ± 30.8 | 961.0 ± 19.2 | |
| 11 | 907.0 ± 39.5 | 1019.8 ± 11.4 | 878.9 ± 45.9 | |
| 12 | 826.9 ± 33.0 | 999.3 ± 11.6 | 912.5 ± 141.7 | |
| 14 | 913.2 ± 81.8 | 1240.1 ± 20.7 | 982.0 ± 70.3 | |
| 15 | 822.7 ± 16.3 | 984.0 ± 37.2 | 794.7 ± 30.1 | |
| 17 | 785.7 ± 28.7 | 989.5 ± 3.7 | 730.0 ± 43.5 | |
| Physiological Parameter | Age of Culture (Days) | Mean ± SE | Mean ± SE | Mean ± SE |
| P. delicatissima | ||||
| D0 | DD | DM | ||
| Percentage of Active Cells | 1 | 96.0 ± 0.5 | 95.4 ± 1.3 | 95.3 ± 1.5 |
| 2 | 98.9 ± 0.3 | 98.4 ± 0.4 | 98.7 ± 0.3 | |
| 3 | 99.6 ± 0.2 | 99.2 ± 0.2 | 99.1 ± 0.2 | |
| 4 | 99.5 ± 0.1 | 99.0 ± 0.4 | 99.2 ± 0.0 | |
| 6 | 99.4 ± 0.2 | 98.6 ± 0.1 | 98.9 ± 0.1 | |
| 7 | 99.3 ± 0.1 | 99.0 ± 0.1 | 99.0 ± 0.0 | |
| 8 | 98.9 ± 0.1 | 98.4 ± 0.0 | 98.5 ± 0.1 | |
| 9 | 97.5 ± 0.1 | 94.9 ± 0.0 | 86.7 ± 0.5 | |
| D0 | DD | DM | ||
| Esterase Activity | 1 | 33.6 ± 3.0 | 37.9 ± 2.4 | 35.1 ± 2.3 |
| 2 | 46.5 ± 0.4 | 51.1 ± 4.6 | 52.0 ± 3.4 | |
| 3 | 33.9 ± 0.2 | 40.9 ± 1.0 | 38.8 ± 2.0 | |
| 4 | 34.9 ± 1.8 | 37.8 ± 4.5 | 40.5 ± 0.8 | |
| 6 | 40.1 ± 4.4 | 33.1 ± 0.8 | 45.6 ± 5.4 | |
| 7 | 47.4 ± 0.2 | 38.2 ± 1.7 | 60.2 ± 2.0 | |
| 8 | 33.4 ± 1.6 | 38.9 ± 1.4 | 73.0 ± 15.7 | |
| 9 | 34.2 ± 1.6 | 45.4 ± 1.0 | 59.5 ± 9.5 | |
| D0 | DD | DM | ||
| Lipid Content | 1 | 108.5 ± 20.9 | 102.7 ± 5.3 | 105.6 ± 4.2 |
| 2 | 124.9 ± 13.6 | 138.4 ± 27.3 | 128.8 ± 23.9 | |
| 3 | 116.3 ± 3.7 | 151.9 ± 7.4 | 135.8 ± 4.4 | |
| 4 | 124.9 ± 4.3 | 161.8 ± 4.6 | 152.0 ± 15.3 | |
| 6 | 150.3 ± 11.6 | 227.6 ± 10.7 | 184.2 ± 9.6 | |
| 7 | 95.5 ± 13.2 | 158.6 ± 8.3 | 148.8 ± 5.5 | |
| 8 | 118.9 ± 17.3 | 143.7 ± 1.6 | 171.8 ± 3.5 | |
| 9 | 207.7 ± 10.6 | 199.8 ± 5.6 | 242.9 ± 4.7 | |