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. Author manuscript; available in PMC: 2014 Jun 26.
Published in final edited form as: Sci Transl Med. 2013 Sep 18;5(203):203ra125. doi: 10.1126/scitranslmed.3006061

Figure 4.

Figure 4

Phosphopeptide-specific immunity is present in healthy individuals within the circulating memory T-cell compartment. (a) Marked heterogeneity of phosphopeptide-specific immunity in 10 healthy donors against 86 antigens revealed by 7-day ELISpot assays. (b) ELISpot results for immunodominant phosphopeptides directly ex vivo and following 14-days in vitro expansion when tetramer binding is evident. (c) Expanded T-cells do not recognize the unphosphorylated counterpart peptides. (d) The relative magnitude of phosphopeptide-specific T-cells compared against common viral epitopes following overnight culture (n=3). (e) Analysis of memory-flow-sorted CD8+ enriched T-cells by 7-day ELISpot reveal phosphopeptide-specific responses reside in the memory compartment.

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