Figure 5.

Effect of ethyl acetate fraction of M. tenuiflora against T. serrulatus venom-induced inflammation. BALB/c mice were treated intravenously (i.v.) with EtOAc fractions (a) at doses of 20, 30, and 40 mg/kg and a few minutes later were injected i.p. with 0.1 mL of VTs (0.8 mg/kg). After six hours, peritoneal lavage was performed with PBS and the cell number was determined in a Neubauer chamber. The supernatants were collected for determination of IL-6 (b), IL-12 (c), and IL-1β (d) levels, which was performed using an enzyme-linked immunosorbent assay. N = 6 , *P ≤ 0.005, compared to PBS and drug-treated groups.