Figure 6.

Cross-eyed stereo view of the conserved cleft between the two domains in the BT2193 structure. Surface and individual residues are coloured on a spectrum according to ConSurf [44] conservation values with blue indicating conservation and red its lack. Putative catalytic acidic residues are shown in ball and stick, as is the nearby bound glycerol molecules in white whose hydrogen bonds to the protein are shown as dotted lines. Surface-exposed Tyr and Trp residues, mainly well-conserved, and an additional highly conserved residue Asn334 and are shown in a stick representation. The region matching the domain-defining sequence motif GxGYxYP (391 GSSYIFP 397 here) is shown as a magenta tube and bears one of the conserved solvent-exposed aromatic residues, Tyr394.