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AtPEN-2 is partially localized to autophagosome vesicles in Arabidopsis protoplasts. (A) AtPEN-2 colocalizes with AtATG8, protein marker for autophagosome in plants (white arrow). However, a set of vesicles with AtPEN-2–GFP signal alone could be seen (green arrow) indicating existence of two populations of AtPEN-2 protein. (B) AtPEN-2 did not show colocalization when AtATG8 was not recruited to autophagosome membranes and signal was present in cytoplasm. Bar, 10 µm.
