Table 3.

Colocalization of particular γ-secretase subunits, average fluorescence lifetimes of donor proteins, donor proteins in the presence of potential acceptor and FRET efficiency of distinct FRET pairs. SD, standard deviation

Donor protein	Acceptor protein	Pearson’s correlation coefficient ±SD	Donor lifetime TD (ns)±SD	Donor lifetime in the presence of potential acceptor TDA (ns)±SD	FRET efficiency, EFRET (%)
eGFP	mRFP	0.95±0.02	2.43 ns±0.03	2.31 ns±0.04	5%
AtPS1–GFP	AtAPH-1–RFP	0.83±0.04	2.39 ns±0.02	1.94 ns±0.03	19%
AtAtPS2–GFP	AtAPH-1–RFP	0.91±0.03	2.39 ns±0.05	1.91 ns±0.09	20%
AtPEN-2–GFP	AtAPH-1–RFP	0.72±0.03	2.34 ns±0.02	2.33 ns±0.11	0.5%
AtNCT–GFP	AtAPH-1–RFP	0.84±0.03	2.36 ns±0.12	2.04 ns±0.04	14%
AtPS1–GFP	AtNCT–RFP	0.85±0.02	2.39 ns±0.02	1.93 ns±0.02	19%
AtPEN-2–GFP	AtNCT–RFP	0.81±0.05	2.34 ns±0.02	2.00 ns±0.07	11%
AtPS1–GFP	AtPEN-2–RFP	0.91±0.05	2.39 ns±0.02	1.81 ns±0.12	24%
AtPS2–GFP	AtPEN-2–RFP	0.78±0.05	2.39 ns±0.05	1.63 ns±0.22	32%
AtPEN-2N74L–GFP	AtPS2–RFP	0.89±0.04	2.36 ns±0.03	2.34 ns±0.03	1%