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. 2014 Apr 10;65(12):3055–3069. doi: 10.1093/jxb/eru150

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — OsNMD3^ΔNLS interfered with the release of OsNMD3 from the 60S subunit. (A) Ribosomal extracts from wild-type and L1 plants (OsNMD3 ^ΔNLS GFP) were fractionated on a 5–50% sucrose density gradient; A ₂₆₀ peaks representing free 40S and 60S subunits, 80S monosomes, and polysomes are indicated. (B and C) Fractions from the gradient shown in A were collected and subjected to immunoblotting with the indicated antibodies; RPL4 and RPS14 were used as the markers for the 60S and 40S subunits, respectively; red and blue asterisks indicate exogenous and endogenous OsNMD3, respectively (this figure is available in colour at JXB online).