Skip to main content
. 2014 Apr 10;65(12):3055–3069. doi: 10.1093/jxb/eru150

Fig. 7.

Fig. 7.

The translational efficiency of plants expressing OsNMD3 ΔNLS GFP is reduced. (A) Transactivation assay of GAL4-VP16 in protoplast cells isolated from wild-type and L1 plants. (B) Relative expression levels of genes implicated in cell-wall biosynthesis in wild-type and two transgenic lines (left panel); data are mean±SE (n=3, *P<0.01 by Student’s t-test); protein blots were probed with rice CESA-specific antibodies; total membrane proteins were extracted from wild-type and two transgenic lines (right panel); molecular weights (kDa) are indicated. (C) Cell-wall composition of the internodes of the wild-type and two transgenic lines. (D) Pharmaceutical treatment of 4-d-old wild-type and L1 seedlings was performed in media containing different concentrations of antibiotics and chemicals for 6 d; relative freshweight was determined by considering the weight of untreated seedlings as the reference. Data are mean±SE (n=30, *P<0.01 by Student’s t-test) (this figure is available in colour at JXB online).