Fig. 5.

Histochemical staining by NBT (A–E) and determination of the production rate (F) of superoxide radicals in lettuce seeds imbibed in water (A–C) or 0.3% SDIC (D, E). (A, D) CAPs. (B, E) RADs. (C) Longitudinal section of RADs. Data are means ±SE of three biological replicates of 300 CAPs or RADs each. Significant differences in the data at 16, 18, and 22h from those at 14h of treatment in water, and at 22, 28, and 34h from those at 18h of treatment in 0.3% SDIC were assessed by Student’s t-test (*P<0.05, **P< 0.01). (This figure is available in colour at JXB online.)