Fig. 3.

Transient expression and localization of the phot2 kinase domain. (A, B) Kinase-inactive phot2 (35S-phot2D720/N–GFP) transiently expressed in N. benthamiana epidermal cells and protoplasts. 0min represents images from the dark-adapted samples. (C) Kinase domain (534–915 amino acids, 35S-phot2C–GFP) or kinase-inactive domain (35S-phot2C-D720/N–GFP) transiently expressed in Nicotiana epidermal cells. All cells were irradiated with a blue laser beam which was also used for excitation of GFP.