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Effect of BFA and wortmannin (WM) on formation of phot2-labelled punctuate structures. (A–D) Nicotiana epidermal cells expressing 35S-phot2–GFP. (A) In the presence of 10 μM BFA, a criss-cross pattern was visible in the epidermal cells after irradiation with a blue laser (10%) for 5min. (B) Removal of BFA restored the formation of phot2 punctuate structures after blue light irradiation. (C, D) Application of WM had no inhibitory effect on blue-light-induced phot2 punctuate structures.
