Fig. 4.

Persistence of the SA-mediated antagonistic effect on JA-induced repellency to spider mites: first choice of female spider mites in 15 min-two-choice behavioural assays offering leaf disks cut from Lima bean plants just before the experiment. These experiments were repeated three times at three different days, each with 20 mites; thus, a total of 60 mites were individually used in each treatment (n=60; data of the individual replicates are provided in Table S1). Ctrl: the plants were sprayed with the solutions of either 0.001% or 1% ethanol as negative controls for 0.001mM SA- and 1mM SA-treatments respectively. Data points located in areas above or below dashed lines indicated by arrows designated ‘attraction’ or ‘avoidance’ indicate a choice distribution significantly different from 50:50 (α=0.05, χ² test) (see Supplementary Table S1 for details of the statistical analyses).