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Dosage response of PvSPX genes to P deficiency. Seedlings were grown in nutrient solution supplied with 5, 50, 100, or 500 μM KH2PO4. After 10 d, total RNA was isolated from leaves and roots for qPCR analysis. Expression levels of the tested genes were quantified relative to expression levels of the reference gene EF-1α (PvTC3216) using arbitrary units. Each bar is the mean of four replicates with the standard error. Different letters represent significant differences at the 0.05 level.
