Fig. 7.

Transcription levels of downstream genes of PvSPX1 in CK and PvSPX1-overexpressing bean hairy roots. Expression patterns of downstream genes were determined by qPCR in CK and two PvSPX1 overexpression hairy root lines grown in MS medium containing 1.25mM P. Expression levels of the tested genes were quantified relative to expression levels of the reference gene EF-1α (PvTC3216) using arbitrary units. OX1 and OX2 indicate two transgenic bean hairy root lines overexpressing PvSPX1. CK indicates the transgenic line transformed with the empty vector. Asterisks represent significant differences of downstream gene expression levels between PvSPX1-overexpressing and CK in t-tests. *0.01<P≤0.05; **0.001<P≤0.01; ***P≤0.001.