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Transformation efficiency of embryogenic calli derived from immature embryos of IR64 rice infected with A. tumefaciens LBA4404 containing ZFN constructs.
| Constructs | Immature embryos | Resistant calli (%) | Regenerated calli (%) | GUS-positive plants | Transformation efficiency (%) |
|---|---|---|---|---|---|
| pZFN1 | 200 | 85.5 | 32.2 | 29 | 27.5 |
| pZFN2 | 150 | 88.7 | 49.6 | 60 | 44.0 |
| pZFN3 | 300 | 146.3 | 45.3 | 188 | 66.3 |
