Fig. 7.

β4-spectrin expression starts being detected at E11.5 and is not required for the early AIS assembly. a–c Immunolocalization of AnkG (with rabbit anti-AnkG) (a1–c1) and β4-spectrin (a2–c2) was analyzed in spinal cord (delimited by dashed lines) and in ventral roots (arrowheads) at E10.5 (a), E11.5 (b) and E13.5 (c). d–e AnkG distribution (with rabbit anti-AnkG) was similar in β4-spectrin ^−/− (d2, e2) and WT embryos (d1, e1) both in the ventral spinal cord and in the ventral root at E12.5 (d1, d2) and E13.5 (e1, e2). AnkG IF profile along the ventral root (d3, e3, n = 3) in WT and β4-spectrin ^−/− embryos at E12.5 (d3) and E13.5 (e3). PanNav/AnkG IF intensities in the ventral root at E12.5 (d4, n = 3; 16 paths drawn) and within the spinal cord at E13.5 (e4, n = 3; 15 fibers measured). Scale bar represent 25 μm