Figure 2.

Nest-GFP+ cells in the peripheral blood and BM of Nest-GFP mice. (A) Nest-GFP transgenic mice were lethally irradiated with a split dose of 7 + 3 Gy total body irradiation and subsequently adoptively transferred with 2 × 10⁶ murine wt BM cells from C57BL/6 donor mice into the tail vain (NestwtBM) and vice-versa (wtNestBM). One group of irradiated animals received NestBM for hematopoietic reconstitution which was supplemented with cultured EGFP(+) MSCs derived from the bone-marrow of C57BL/6-Tg(CAG-EGFP)1Osb/J mice [NestBM + EGFP(+) MSC]. After hematopoietic reconstitution (6–8 weeks after transplantation) blood samples were taken, erythrocytes were removed, and analyzed for GFP-expression by immunofluorescence. Lymphocytes are stained in red. (B) Paraffin-sections of Nest-GFP BM specimen were stained for GFP-expression. (C) The blood analysis showed a complete engraftment of donor BM by the donor BM-derived Nest-GFP transplanted progenitor cells and vice-versa as indicated by GFP-expression. Control, normal Nest-GFP transgenic mice.