Adult DRG neurons co-transfected with Pea3 expression vector and DsRed and stimulated with bFGF, EGF, or IGF-1. (A) representative images of DRG neurons co-transfected with Pea3 and DsRed, stimulated with IGF-1; (B) total axonal growth in adult DRG neurons transfected with DsRed alone (left panel; IGF-1 and bFGF compared with control, using one way ANOVA test with Tukey’s post test, **p < 0.01, ***p < 0.001), or co-transfected with DsRed and mPea3, in the presence or absence of growth factors (right panel; EGF, IGF-1, and bFGF compared with control, using one-way ANOVA test with Tukey’s post test, **p < 0.01, ***p < 0.001); (C) difference in maximal axonal outgrowth normalized to control cells transfected with DsRed alone (left panel; IGF-1 and bFGF compared with control; using one way ANOVA test with Tukey’s post test, *p < 0.01, **p < 0.001), or co-transfected with DsRed and mPea3, in the presence or absence of growth factors (right panel; EGF, IGF-1, and bFGF compared with control, using one-way ANOVA test with Tukey’s post test, **p < 0.01, ***p < 0.001); (D) number of branch points in adult DRG neurons transfected with DsRed alone (left panel; IGF-1 and bFGF compared with control; using one way ANOVA test with Tukey’s post test, **p < 0.01, ***p < 0.001), or co-transfected with DsRed and mPea3, in the presence or absence of growth factors (right panel; EGF, IGF-1 and bFGF compared with control, using one-way ANOVA test with Tukey’s post test, **p < 0.01, ***p < 0.001).