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. 2014 Jun 26;7:59. doi: 10.3389/fnmol.2014.00059

FIGURE 3.

FIGURE 3

Pea3 can enhance axonal outgrowth in NSC-34 motor neuron cell lines. (A) Percent differentiation as scored by the number of green fluorescent cells with axonal outgrowth longer than 2 cell body length versus total GFP-expressing cells (two sets of duplicates are shown, at least four different fields are scored per well); (B) representative images of NSC-34 cells transfected with either empty pCDNA3 vector, pCDNA3-mPea3, pCDNA3-VP16, pCDNA3-mPea3-VP16 or pCDNA3-mPea3-EN; (C) Percent differentiation by Pea3 phosphorylation mutants as scored by the number of green fluorescent cells with axonal outgrowth longer than two cell body length versus total GFP-expressing cells (two sets of duplicates are shown, at least four different fields are scored per well); (D) representative images of NSC-34 cells transfected with either empty pCDNA3 vector, wildtype pCDNA3-mPea3, or the phosphorylation mutants pCDNA3-mPea3-S90A, pCDNA3-mPea3-S90E.