FIGURE 4.

Neurofilament-L and –M promoters contain several ets motifs with significant similarity to Pea3 consensus binding site. (A) Human Neurofilament-L and –M promoters were analyzed by with Promo 3.0 (http://alggen.lsi.upc.es/cgi-bin/promo_v3/promo/promoinit.cgi?dirDB=TF_8.3), and dissimilarity rates (which indicate the variance between the binding motif of the transcription factor and the nucleotide sequence on the promoter with respect to predicted transcription start site, shown as percentage with respect to the binding matrices) are written below the predicted ets motifs; (B) the expression analysis of NF-L and pea3 genes in SH-SY5Y cells stimulated with IGF-1 for 30 and 60 min; (C) the expression analysis of NF-M, NF-L, pea3 and gapdh genes in SH-SY5Y cells before (day 0) or after (days 1 and 3) of stimulation in the presence (+) or absence (-) of Retinoic acid and aphidicolin (RA/aph) as described in Sections “Materials and Methods.”