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. 2014 Jun 26;7:59. doi: 10.3389/fnmol.2014.00059

FIGURE 6.

FIGURE 6

Neurofilament-M as a potential target promoter for activation by Pea3. (A) hNF-M promoter, which contains 1 major ets motif, was cloned upstream of a luciferase reporter gene; (B) luciferase reporter gene expression assay with either wild type 200 ng of NFM promoter or NFM-Δ with deletion of putative Pea3 transcription factor binding site, in SH-SY5Y cells (luciferase activities represent reproducible three to four independent experiments and data plotted for each condition were average of relative luciferase activity of triplicate samples); (C) quantitative PCR results of chromatin immunoprecipitation (ChIP) assay, where Flag-tagged mPea3 expression plasmid was co-transfected into the HEK293 cells with or without NFM wild type and NFM-Δ luciferase reporter, subjected to chromatin precipitation with anti-Flag antibody and purified DNA material was amplified with NF-M gene promoter primers.