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Dye coupling is confirmed by anti-connexin 35/36 immuno-labeling. (A) AFB-594 and Mini-Emerald retrograde labeling revealed motor neurons from the 14th ventral root, and showed the extensive labeling of presumably coupled neurons. Whole-mount immunohistochemistry with anti-Cx35 antibody confirms the widespread presence of connexins (green puncta) in both male (B) and female (C) Mosquitofish. Calibration bars equals (A) 200 um, (B,C) 50 um.
