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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Anal Biochem. 2013 Oct 23;446:76–81. doi: 10.1016/j.ab.2013.10.024

Fig. 3.

Fig. 3

Percentage of carbamylated peptides from serum proteins digested in different buffers with urea. Human serum proteins were denatured, reduced, alkylated, and digested in different buffers (0.1M PB, 0.2M Tris-HCl, 0.2M NH4HCO3 and 1M NH4HCO3) with urea. The tryptic peptides were analyzed by LC-MS/MS and spectra were assigned by database search. The percentage of peptides assigned to different forms of carbamylated peptides was determined based on spectral counts.