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Possible mechanism of carbamylation inhibition by ammonium containing buffer. (1) The decomposition of urea in aqueous solutions, where k1 and k2 represent the decomposition constant and formation constant of urea, respectively. (2) Carbamylation of proteins or peptides by cyanate. (3) The relationship among ammonium ion and cyanate concentrations and protein carbamylation under conditions of fixed urea solution concentrations and temperature.
