Figure 4.

Geldanamycin (GA) significantly reduced intracellular reactive oxygen species (ROS) generation by 3-nitropropionic acid (3NP). (A) Intracellular level of ROS was measured using confocal microscopy. Striatal cells were treated with GA and incubated in the presence or absence of 3NP. Cells were incubated with fluorescence probe H2DCFDA (10 μM). (B) H2DCFDA fluorescence was quantitatively analyzed with Fluoview 300 software. The production of 3NP-induced ROS was significantly attenuated by GA in striatal cells. Results are the means ± SD of three independent experiments performed in triplicate. ^*P<0.05, ^**p<0.01 indicate significant differences compared to the control. ^##P<0.05 indicates significant differences between the indicated groups.