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. 2014 Apr 22;34(1):24–34. doi: 10.3892/ijmm.2014.1747

Figure 6.

Figure 6

Triptolide (TL) inhibits the expression of heat shock protein (HSP) 70 and abrogates protective effect by geldanamycin (GA). (A) Striatal cells were treated with indicated concentration of TL. After 2 h, cells were harvested for immunoblotting of HSP 70 and HSP 90. Expression of HSP 70 was inhibited in a dose-dependent manner by TL, while no change was observed for HSP 90. β-actin was used as a loading control. TL significantly attenuated the protective effect of GA against 3-nitropropionic acid (3NP)-induced cell death. (B and C) Cell viability was examined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase leakage (LDH) assay. Prior to treatment with 3NP, striatal cells were incubated for 2 h with TL, an inhibitor of HSP 70. TL did not cause cell death at a concentration of 50 nM used in the present study. Data were obtained from four independent experiments (n=4) and expressed as mean ± SD. *P<0.05 and **p<0.01 indicate significant differences compared to the control. #P<0.05 and ##p<0.01 indicates significant differences between the indicated groups.