Table 2. Pharmacokinetic Parameters Derived from Noncompartmental Analysis of 3H-Liposomes Injected into CD-1 Micea.
formulation | t1/2 (h) | AUC0-∞ (%ID·h/mL) | Vd (mL) | CL (mL/h) | MRT (h) |
---|---|---|---|---|---|
5–8 kDa HA | 6.32 ± 0.69 | 327 ± 53 | 2.87 ± 0.77 | 0.31 ± 0.05 | 8.7 ± 0.6 |
50–60 kDa HA | 7.35 ± 0.75 | 324 ± 34 | 3.28 ± 0.21 | 0.31 ± 0.03 | 9.6 ± 1.0 |
175–350 kDa HA | 9.14 ± 1.64 | 216 ± 34* | 6.14 ± 0.98** | 0.47 ± 0.07* | 11.5 ± 3.1 |
mPEG-5000 | 9.60 ± 2.46 | 428 ± 8* | 3.23 ± 0.78 | 0.23 ± 0.01 | 11.5 ± 3.3 |
All values are reported as mean ± SD. AUC0-∞ values were calculated by using trapezoidal integration of radioactivity concentrations (%ID/mL blood) versus time curves from 0 to 24 h and extrapolation from 24 h to infinity. Statistical analyses among HA-liposomes and PEGylated liposomes were conducted using one-way ANOVA followed by Tukey’s multiple comparison test. *p < 0.05; **p < 0.01 significant difference compared to all other groups.