WST-1 cell viability assay. C. albicans cells (A, 10231;
B, MYA-576) were cultured in a microplate with 100 μl of
RPMI-1640 containing varying concentrations of bHK peptides for 48h at room
temperature. At the end of the treatment, 10 μl of WST-1 assay solution
were added to each well, and after 30 mins, the reduced formazan was measured at
450 nm (650nm as reference). Cells without peptide were used as control.
H2K4b-RP represents H2K4b-PEG-cRGD.