Figure 8. Effect of A_2A receptor antagonism on microglial response to tissue damage in slices from MPTP-treated animals.

CX₃CR1^GFP/+ mice were injected with 20 mg/kg s.c. MPTP once daily for 5 days, and slices were prepared for imaging 4-7 days later. The perfusion solution contained either the selective A_2A receptor antagonist preladenant (5 μM) or DMSO vehicle. 2D projections of the optical stacks at each time point were used for analysis by tracking moving objects larger than 2 μm in diameter. The average displacement of all moving objects (A), the mean instantaneous velocity (B), and the fraction of tracks with longer than 5 μm displacement (C) were calculated for baseline (BL) recordings before injury and after the induction of the injury in the same slice. Numbers of slices for each condition are shown in parentheses. Statistics: two-way repeated measures ANOVA and Tukey’s post hoc test. *, p < 0.05.