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. 2014 Jun 26;10(6):e1004407. doi: 10.1371/journal.pgen.1004407

Figure 2. Sequence motifs function “out of context”.

Figure 2

A) Motifs associated with splicing efficiency were revealed by comparing intron sequences of high and low expressing YiFP strains. Examples of five enhancers (enrichment p<3.4e-4) and silencers (p<4e-4) are shown. B) The novel motifs were enriched in proximity to intron ends. Enrichment of motifs in introns compared to a randomized/permutated version of the motifs that maintain their properties (blue line, Materials and methods) is presented in respect to distance from 5′- or 3′ Splice Site (SS) (top and bottom, respectively). Positions significantly enriched or deprived of motifs are marked in red and green, respectively. C) Reporter YFP expression is decreased upon exchange of an enhancer to a silencer motif in two independent intron strains. Mean YFP expression was calculated for triplicates of the two intron library strains (YGL076C- p = 0.02, and YDL064W- p = 0.2). Average expression of each mutated motif strain is shown in comparison to that of the natural intron harboring YiFP strain (100%). Numbers inset in bars indicate the mean YFP expression level (non-relative).