Figure 3. Key pathway changes identified by ingenuity pathway analysis.

(A) The top ten altered canonical signaling pathways. Note that three pathways were related with stem cell functions: embryonic stem cell pluripotency, Nanog, and Wnt. (B) Alteration of myeloid differentiation pathway. The expression changes of all seven genes by array analysis were validated by real time quantitative PCR. “Change direction by references” was compiled by the software for the impact of expression of a gene on myeloid differentiation according to literature. “Prediction” indicated potential impact on differentiation based on array gene expression data. STAP1 gene was re-annotated as Affected/Decreased under “Change direction by references” according to current literature, but the Z score and p value were based on STAP1 annotated as “Affected” in the software.