Figure 9. ATRA treatment affected SIRT1 functions.
(A) FOXO1 acetylation change in response to 1 µM ATRA was analyzed by flow cytometry with an acetylated FOXO1 antibody. (B) Ku70 acetylation change in response to mock or 1 µM ATRA for 24 to 72 h was analyzed by immunoprecipitation with a Ku70 antibody and Western blot with a pan-acetyl lysine antibody. (C) NHEJ and HR reporter assays in KCL-22 cells in response to 1 µM ATRA treatment.