Figure 2. Stereotyped DEVDase activation in the AL and EB structures of aged Drosophila brains.
(A, B, D, E) Representative aged brains (45-days-old) bearing cPARP signals (DEVDase activity) in the dorso-medial side of the AL (A), the EB structure (B), the mushroom body (MB) (D) and the local interneuron (LN) of the AL (E). mCD8::PARP::Venus was expressed in most postmitotic neurons (elav-Gal4;;UAS-mCD8::PARP::Venus). Circles of broken lines are ALs. cPARP signal and mCD8::PARP::Venus expression are shown in magenta and green, respectively. Scale bar: 75 µm. (C) Percentages of brains with cPAPR signal in the EB and AL at each time point are shown. “n” indicates the number of brains examined. Genotypes: (A–E) elav-Gal4;;UAS-mCD8::PARP::Venus.