Figure 6. Zfy2 acidic domain is a much more potent transactivator than other ‘Zf’ acidic domains.

Levels of β-galactosidase induced by the Gal4-DNA-binding domain on its own (pGB-CEN6; negative control) or fused to an acidic domain from one of six different ZF isoforms from human (hs) or mouse (mm). Among the mouse sex-linked genes, Zfy2 has a substantially more potent activation domain than Zfy1, and Zfx is significantly less potent than Zfy1. mm ZFA derives from the autosomal Zfa gene that originated from a retroposed X transcript. *p≤0.05; **p≤0.01.