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. 2014 Jul 1;25(13):1946–1957. doi: 10.1091/mbc.E13-06-0300

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© 2014 Goss et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Primary septum assembly is delayed in blt1∆ mutants. Times are in minutes, with spindle pole body separation at time zero. (A) Time series of fluorescence micrographs at 4-min intervals of wild-type (blt1⁺, top) or blt1∆ (bottom) cells expressing Bgs1p-mEGFP (green) and Sad1p-RFP (red). (B, C) Comparisons of wild-type (open symbols) and blt1∆ (filled symbols) cells. (B) Time courses of (, ) the accumulation of cells with diffuse localization of Bgs1p-mEGFP ±1 SD around the equator (12 min in the blt1⁺ cell in A) and (, ) accumulation of cells with Bgs1p-mEGFP ±1 SD condensed into a ring at the site of primary septum formation (as shown in the blt1⁺ cell in A at 24 min) in 33 wild-type cells and 36 blt1∆ cells. Asterisks indicate time points at which the mean values of the two cell types differed with p < 0.0003. (C) Time courses of the onset of primary septum assembly ±1 SD in () 33 wild-type and () 36 blt1∆ cells. Asterisks indicate time points at which the mean values of the two cell types differed with p < 0.0001. (D) Time course of the mean number ±1 SD of Bgs1p-mEGFP molecules at the cleavage furrow in 20 wild-type cells (black line) and 21 blt1∆ cells (gray line). Asterisks indicate time points at which the mean values of the two cell types differed with p < 0.0001. Scale bar, 5 μm.

Inline graphic — Primary septum assembly is delayed in blt1∆ mutants. Times are in minutes, with spindle pole body separation at time zero. (A) Time series of fluorescence micrographs at 4-min intervals of wild-type (blt1⁺, top) or blt1∆ (bottom) cells expressing Bgs1p-mEGFP (green) and Sad1p-RFP (red). (B, C) Comparisons of wild-type (open symbols) and blt1∆ (filled symbols) cells. (B) Time courses of (, ) the accumulation of cells with diffuse localization of Bgs1p-mEGFP ±1 SD around the equator (12 min in the blt1⁺ cell in A) and (, ) accumulation of cells with Bgs1p-mEGFP ±1 SD condensed into a ring at the site of primary septum formation (as shown in the blt1⁺ cell in A at 24 min) in 33 wild-type cells and 36 blt1∆ cells. Asterisks indicate time points at which the mean values of the two cell types differed with p < 0.0003. (C) Time courses of the onset of primary septum assembly ±1 SD in () 33 wild-type and () 36 blt1∆ cells. Asterisks indicate time points at which the mean values of the two cell types differed with p < 0.0001. (D) Time course of the mean number ±1 SD of Bgs1p-mEGFP molecules at the cleavage furrow in 20 wild-type cells (black line) and 21 blt1∆ cells (gray line). Asterisks indicate time points at which the mean values of the two cell types differed with p < 0.0001. Scale bar, 5 μm.