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. 2014 Jul 1;25(13):2051–2060. doi: 10.1091/mbc.E14-03-0785

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© 2014 Gayek and Ohi. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Kif15 spindle localization is independent of K-MT stability. (A) Kif15 spindle localization is not reduced in HeLa cells depleted of HURP. HeLa cells transfected with control or HURP siRNA were treated with 5 μM MG-132 for 90 min. HURP (yellow), Kif15 (magenta), and tubulin (green) were detected by immunostaining. DNA (blue) was counterstained with Hoechst 33342. LUTs are scaled identically for each channel. Scale bar, 5 μm. (B) Ratio of spindle Kif15 intensity to spindle tubulin intensity after treatment as in A. Box-and-whisker plots display median flanked by 10th, 25th, 75th, and 90th percentiles. Each data set includes at least 30 cells from three experiments. p = 0.86. (C) Kif15 spindle localization is not increased in RPE-1 cells treated with 2.5 nM Taxol. RPE-1 cells were treated with 5 μM MG-132 with or without 2.5 nM Taxol for 90 min. Kif15 (magenta) and tubulin (green) were detected by immunostaining. DNA (blue) was counterstained with Hoechst 33342. LUTs are scaled identically for each channel. Scale bar, 5 μm. (D) Ratio of spindle Kif15 intensity to spindle tubulin intensity after treatment as in C. Box-and-whisker plots display median flanked by 10th, 25th, 75th, and 90th percentiles. Each data set includes at least 30 cells from three experiments. p = 0.22.

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