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. 2014 Jul 1;25(13):2061–2070. doi: 10.1091/mbc.E13-10-0582

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© 2014 Williams et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Localization of SNAP23, Syntaxin4, VAMP7, and MT1-MMP at invadopodia. (A) Cells were plated on 594-labeled gelatin, fixed, permeabilized, and stained for SNAP23, Syntaxin4, or VAMP7 (green) along with MT1-MMP (blue). (B) Cells transfected with MT1-MMP-FLAG were plated on 594-labeled gelatin, fixed, permeabilized, and stained for Syntaxin3 (green) and FLAG epitope (for MT1-MMP, blue). Invadopodia are marked by the colocalization of MT1-MMP and dark spots of degraded gelatin. Scale bar, 10 μm.