TABLE 1:
Analysis of germ cell corpses in animals treated with RNAi of candidate lysosomal cathepsins in C. elegans.
| Human cathepsin | C. elegans cathepsin genes (RNAi) | Number of cell corpses in rrf-3(pk1426) mutants (mean ± SEM) | Number of germ cell corpses in cpl-1(yq89) mutants (mean ± SEM) |
|---|---|---|---|
| Control RNAi | 1.4 ± 0.3 | ||
| Control RNAi | 28.9 ± 1.5 | ||
| Cathepsin A | F13D12.6 | 1.7 ± 0.5 | 26.4 ± 1.7 |
| F32A5.3 | 1.9 ± 0.5 | 25.4 ± 2.2 | |
| F41C3.5 | 2.2 ± 0.3 | 27.4 ± 1.5 | |
| K10B2.2 | 1.8 ± 0.4 | 30.1 ± 1.7 | |
| Y16B4A.2 | 1.9 ± 0.5 | 29.3 ± 1.6 | |
| Y32F6A.5 | 1.7 ± 0.2 | 30.1 ± 1.9 | |
| K10C2.1 | 1.7 ± 0.4 | 33.9 ± 1.7 | |
| Y40D12A.2 | 2.6 ± 0.3 | 29.6 ± 2.2 | |
| Cathepsin D | asp-1(Y39B6A.20) | 2.9 ± 0.4 | 32.5 ± 1.8 |
| asp-3(H22K11.1) | 1.8 ± 0.4 | 28.7 ± 2.4 | |
| asp-4(R12H7.2) | 1.5 ± 0.5 | 32.9 ± 2.1 | |
| Cathepsin B | cpr-1(C52E4.1) | 1.1 ± 0.3 | 29.1 ± 1.7 |
| cpr-2(F36D3.9) | 1.4 ± 0.2 | 27.8 ± 2.3 | |
| cpr-3(T10H4.12) | 1.8 ± 0.4 | 32.3 ± 2.2 | |
| cpr-4(F44C4.3) | 1.7 ± 0.4 | 32.4 ± 1.6 | |
| cpr-5(W07B8.5) | 2.3 ± 0.4 | 30.1 ± 1.5 | |
| cpr-6(C25B8.3) | 2.2 ± 0.3 | 30.3 ± 2.2 | |
| F32H5.1 | 2.3 ± 0.4 | 32.9 ± 2.0 | |
| F57F5.1 | 2.5 ± 0.3 | 30.7 ± 2.0 | |
| W07B8.1 | 2.0 ± 0.2 | 27.6 ± 2.4 | |
| W07B8.4 | 2.0 ± 0.3 | 29.0 ± 2.5 | |
| Y65B4A.2 | 1.9 ± 0.2 | 27.9 ± 1.9 | |
| Cathepsin E | asp-5(F21F8.3) | 2.6 ± 0.5 | 29.1 ± 2.3 |
| asp-6(F21F8.7) | 2.1 ± 0.3 | 30.1 ± 1.7 | |
| asp-9(C11D2.2) | 1.7 ± 0.5 | 31.0 ± 1.9 | |
| asp-10(C15C8.3) | 2.1 ± 0.4 | 27.2 ± 1.9 | |
| asp-12(F21F8.4) | 2.2 ± 0.4 | 32.4 ± 2.6 | |
| asp-19(ZK384.6) | 1.6 ± 0.3 | 33.4 ± 1.7 | |
| Cathepsin F | R07E3.1 | 1.7 ± 0.3 | 31.1 ± 2.3 |
| R09F10.1 | 3.0 ± 0.6 | 24.7 ± 1.6 | |
| F41E6.6 | 1.7 ± 0.3 | 29.2 ± 1.6 | |
| Cathepsin H | K02E7.10 | 2.3 ± 0.6 | 29.8 ± 2.1 |
| tag-329(C50F4.3) | 2.5 ± 0.3 | 31.2 ± 1.9 | |
| Cathepsin L | Y51A2D.1 | 2.3 ± 0.6 | 30.4 ± 1.5 |
| Y51A2D.8 | 2.3 ± 0.4 | 26.9 ± 1.4 | |
| Y71H2AR.2 | 2.4 ± 0.4 | 30.4 ± 2.0 | |
| cpl-1(T03E6.7) | 25.0 ± 1.6 | 29.1 ± 2.1 | |
| Cathepsin S | C32B5.7 | 1.4 ± 0.2 | 29.4 ± 2.2 |
| F15D4.4 | 2.8 ± 0.6 | 26.8 ± 2.5 | |
| Y40H7A.10 | 1.9 ± 0.2 | 33.1 ± 1.6 | |
| Y71H2AM.25 | 1.4 ± 0.3 | 29.8 ± 1.3 | |
| Cathepsin Z | cpz-1(F32B5.8) | 1.3 ± 0.3 | 32.0 ± 1.9 |
| cpz-2(M04G12.2) | 1.7 ± 0.3 | 28.8 ± 1.5 |
Germ cell corpses were analyzed in rrf-3(pk1462) adult animals (24 h post L4) grown on RNAi plates at 20°C and in cpl-1(yq89) animals that were grown to L4 molt on RNAi plates at 20°C and then transferred to 25°C for further culture of 24 h. The identity of each RNAi clone was confirmed by sequencing.