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. 2014 Jun 26;9(6):e98515. doi: 10.1371/journal.pone.0098515

Figure 4. FGFR2 inhibition causes cell death in NCI-H716 cells.

Figure 4

A. Lysates from Figure 3B reveal increased PARP cleavage. Phospho S6RP is included as reference and GAPDH was used as a loading control. B. Cell cycle profile of treated cells. 1×10exp6 NCI-H716 cells were treated with 100 nM PD173074, MEK inhibitor (100 nM) or MEK + AKT inhibitor (L-547, 1 uM) or DMSO (untreated) were isolated at the indicated time points and processed for Propidium Iodide staining and FACS analysis as described in Materials and methods. B. indicates a tabular representation of cell cycle profiles as determined by manual gating for G1, S, G2/M and subG1 areas. C is the cell cycle profiles.