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. 2014 Jun 26;10(6):e1004465. doi: 10.1371/journal.pgen.1004465

Figure 1. Generation of a murine model of Cyclophilin B deficiency.

Figure 1

(A) Left, Diagram of knock-out allele containing β-geo reporter construct inserted into intron 1 of Ppib gene. Hemi-nested primers (arrows) for PCR-based genotyping selectively amplify the wild-type allele (255 bp) or the gene-trapped allele (364 bp). Right, Genotypes in offspring of matings between mice heterozygous for the gene-trapped allele are noted as wild-type (+/+), heterozygous (+/−) or homozygous (−/−). (B) Verification of Ppib expression by Real-time RT-PCR using total RNA isolated from primary cultures and tissues from newborns and 8-week mice, respectively. Values represent the average of two independent fibroblast and osteoblast cultures, and five independent dermal and femoral samples. (C) Lengths of femora and tibiae from 8 week-old wild-type (+/+), heterozygous (+/−) and homozygous (−/−) knock-out mice. Rhizomelia was not detected. (D) Growth curves of male and female mice from weaning (4 weeks) to 24 weeks of age.