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. 2014 Jun 26;10(6):e1004465. doi: 10.1371/journal.pgen.1004465

Figure 8. Expression of ER resident collagen helical lysine modification enzymes.

Figure 8

(A–C) Real-time RT-PCR of total RNA from two independent cell cultures of newborn fibroblasts (FB) and calvarial osteoblasts (OB), and 5 independent skin and femoral samples for each genotype at 8 weeks of age. (D) Western blots of cell lysates probing for lysyl hydroxylase 1 (LH1/PLOD1), lysyl hydroxylase 3 (LH3/PLOD3), glycosyltransferase 25 domain containing 1 (GLT25D1), and β-actin in wild-type (+/+), heterozygous (+/−) and homozygous (−/−) Ppib-null cells and tissues.