Table 2. Post-translational modification of type I collagen lysine residues.
| BONE | OB | |||||
| +/+ | +/− | −/− | +/+ | −/− | ||
| α1(I) K87 | Lys | 2.0±1.0 | 2.0±0.4 | 43.0±6.0* # | 0.1±0.1 | 21.2±6.4* |
| Hyl | 6.0±2.0 | 13.0±5.0 | 7.0±1.0# | 13.4±4.8 | 10.1±4.7 | |
| G-Hyl | 24.0±3.0 | 22.0±7.0 | 6.0±6.0* # | 0.9±0.3 | 0.8±0.1 | |
| GG-Hyl | 68.0±4.0 | 63.0±11.0 | 44.0±12.0* # | 85.6±4.6 | 67.7±1.8* | |
| α1(I) K174 | Lys | 18.0±1.0 | 19.0±3.0 | 15.0±2.0 | 15.5±2.7 | 9.3±1.9* |
| Hyl | 67.0±2.0 | 66.0±2.0 | 56.0±1.0* # | 54.2±4.2 | 33.4±1.4* | |
| G-Hyl | 12.0±2.0 | 13.0±1.0 | 19.0±1.0* # | 9.7±3.4 | 8.9±2.9 | |
| GG-Hyl | 2.0±0.0 | 2.0±0.0 | 10.0±2.0* # | 20.5±3.5 | 48.3±2.9* | |
| α1(I) K564 | Lys | 0.0 | 0.3±0.2 | 0.0 | 11.0±5.6 | 9.8±2.4 |
| Hyl | 76.0±4.0 | 79.0±3.0 | 45.0±5.0* # | 56.3±1.3 | 44.9±4.9* | |
| G-Hyl | 21.0±4.0 | 17.0±2.0 | 31.0±3.0# | 13.7±2.5 | 13.4±2.6 | |
| GG-Hyl | 3.0±1.0 | 4.0±1.0 | 24.0±4.0* # | 18.9±3.0 | 31.9±4.7* | |
| α1(I) K603 | Lys | 42.0±3.0 | 40.0±1.0 | 37.0±2.0 | 42.0±4.6 | 28.2±4.3* |
| Hyl | 57.0±3.0 | 59.0±1.0 | 58.0±2.0 | 49.1±5.4 | 48.0±4.8 | |
| G-Hyl | 0.6±0.1 | 0.6±0.0 | 2.0±0.0* | 1.9±0.1 | 4.1±0.6* | |
| GG-Hyl | 0.2±0.1 | 0.4±0.0 | 2.0±1.0* # | 7.0±1.4 | 19.6±2.1* | |
| α1(I) K756 | Lys | 40.0±2.0 | 41.0±1.0 | 41.0±5.0 | 40.5±6.8 | 31.5±3.4 |
| Hyl | 59.5±3.0 | 58.5±0.8 | 57.0±6.0 | 54.5±6.9 | 57.1±1.9 | |
| G-Hyl | 0.4±1.0 | 0.5±0.2 | 1.5±1.0 | 1.2±0.1 | 2.3±0.3* | |
| GG-Hyl | 0.1±0.1 | 0.1±0.1 | 0.6±0.5 | 3.7±0.2 | 10.8±0.9* | |
| α2(I) K87 | Lys | 23.0±5.0 | 20.0±4.0 | 55.0±4.0* # | 0.6±0.1 | 21.3±3.1* |
| Hyl | 77.0±5.0 | 80.0±4.0 | 45.0±4.0* # | 88.9±0.7 | 61.6±3.0* | |
| G-Hyl | 0.0 | 0.0 | 0.0 | 0.3±0.1 | 0.3±0.1 | |
| GG-Hyl | 0.0 | 0.0 | 0.0 | 10.2±0.6 | 16.8±0.4* | |
| α2(I) K174 | Lys | 25.0±3.0 | 20.0±8.0 | 38.0±6.0* # | 24.1±3.0 | 42.0±2.4* |
| Hyl | 19.0±3.0 | 20.0±2.0 | 8.0±3.0* # | 15.3±2.1 | 4.1±0.6* | |
| G-Hyl | 51.0±3.0 | 53.0±6.0 | 38.0±4.0* # | 23.4±2.3 | 8.9±0.6* | |
| GG-Hyl | 5.0±3.0 | 7.0±2.0 | 16.0±2.0* # | 37.3±2.3 | 45.0±2.1* | |
| α2(I) K219 | Lys | 9.0±2.0 | 8.0±2.0 | 8.0±2.0 | 13.3±0.6 | 7.8±0.6* |
| Hyl | 86.0±1.0 | 86.0±2.0 | 75.0±3.0* # | 73.6±5.8 | 50.9±1.0* | |
| G-Hyl | 4.0±1.0 | 4.0±1.0 | 5.0±1.0 | 0.8±0.3 | 1.2±0.3 | |
| GG-Hyl | 1.0±0.6 | 2.0±0.0 | 12.0±4.0* # | 12.3±5.9 | 40.1±1.6* | |
| α2(I) K933 | Lys | 0.0 | 0.0 | 38.0±3.0* # | 2.9±0.2 | 0.0* |
| Hyl | 100.0 | 100.0 | 62.0±3.0* # | 92.0±2.8 | 91.2±1.9 | |
| G-Hyl | 0.0 | 0.0 | 0.0 | 1.0±0.4 | 0.9±0.2 | |
| GG-Hyl | 0.0 | 0.0 | 0.0 | 4.0±2.4 | 7.9±1.7 | |
*p<0.05 between +/+ and −/−;
#
p<0.05 between +/− and −/−;
no significant difference between +/+ and +/− in bone.