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. 2014 Jun 13;14:435. doi: 10.1186/1471-2407-14-435

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Copyright © 2014 Elfineh et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In situ PLA for visualization of total pTyr levels. K562 cells probed in in situ PLA with anti-pTyr antibodies 4G10 (panel A) and PYKD1 (panel B) compared to negative control (panel C) where the primary antibody omitted. Red dots represent detected pTyr proteins, while cell nuclei are stained in blue. Scale bars 10 μm.