Table 2.

Effects of selective P2X ₇ antagonists at GOF and LOF P2X ₇ variants

	Yo-Pro-1 uptake (IC 50 , nM)				Ca 2+ flux (IC 50 , nM)
	WT	155Tyr	348Thr	496Ala	WT	155Tyr	348Thr	496Ala
A-804598	21.77 ± 2.8	21.50 ± 5.8	15.5 ± 0.6	ND	20.81 ± 7.6	19.36 ± 6.9	40.7 ± 14.2	25.57 ± 1.6
A740003	332.83 ± 12.3	449.2 ± 12.7	415.9 ± 98.2	ND	507.87 ± 91.1	379.43 ± 135.1	720.75 ± 27.9	405.50 ± 81.7
AZ11645373	198.2 ± 42	342.6 ± 48.3	218.75 ± 113.5	ND	296.3 ± 98.5	510 ± 39.9	806.75 ± 249.3	412.40 ± 1.7

IC₅₀ values reported here represent the averages ± SEM of 3 separate experiments. For simplicity Hill slope were not included in the table. Data for Yo-Pro-1 uptake assay for 496Ala variant could not be determined (ND) due to the low amplitude of responses obtained with this variant.