Figure 1. Deletion of both Ets1 and Ets2 ablates Hras^G12V transformation of MEFs.

E1+E2+ and E1−E2− MEFs were infected with HRasv^G12V or empty vector control retrovirus, then selected with Hygromycin for at least 5 days before functional examination of the four generated cellular genotypes, as shown. A) Western blotting analysis of 20 µg protein lysates probed with antibodies against proteins shown, with α-tubulin as loading control. B) Representative images and C) Quantification of the cellular colonies that grew in soft agar from the four different genetic groups Asterisk indicates P<0.05 as determined by the Student t test. D) Cells were injected subcutaneously into nude mice (10⁶ cells per injection site) and after 3 weeks the tumors were harvested. The ratios represent the percentage of tumors that grew from the total number of injections for each of the different genotypes.